Expression of the P-glycoprotein gene in multidrug-resistant Chinese hamster ovary cells.

Abstract

A series of multidrug-resistant (MDR) Chinese hamster ovary (CHO) cells was established to survive in stepwise increasing concentrations of vincristine. These MDR cells contained amplified P-glycoprotein (mdr) genes. Using monoclonal antibody against the P-glycoprotein, we present here results showing that the levels of P-glycoprotein and its phosphorylation and glycosylation did not correlate with those of drug resistance. Our results suggest that overproduction of the P-glycoprotein cannot account for the differences in drug resistance in these MDR cells, and that multiple modalities are involved in multiple drug resistance in mammalian cells.

Cite this paper

@article{Mukhopadhyay1989ExpressionOT, title={Expression of the P-glycoprotein gene in multidrug-resistant Chinese hamster ovary cells.}, author={Tapas Kr Mukhopadhyay and Marcus Tien Kuo}, journal={Anticancer research}, year={1989}, volume={9 3}, pages={575-8} }