Expression of the Murine Norovirus (MNV) ORF1 Polyprotein Is Sufficient to Induce Apoptosis in a Virus-Free Cell Model

@article{Herod2014ExpressionOT,
  title={Expression of the Murine Norovirus (MNV) ORF1 Polyprotein Is Sufficient to Induce Apoptosis in a Virus-Free Cell Model},
  author={Morgan R. Herod and Omar Salim and Rachel J. Skilton and Cynthia A. Prince and Vernon K Ward and Paul R. Lambden and Ian N Clarke},
  journal={PLoS ONE},
  year={2014},
  volume={9}
}
Investigations into human norovirus infection, replication and pathogenesis, as well as the development of potential antiviral agents, have been restricted by the lack of a cell culture system for human norovirus. To date, the optimal cell culture surrogate virus model for studying human norovirus biology is the murine norovirus (MNV). In this report we generate a tetracycline-regulated, inducible eukaryotic cell system expressing the entire MNV ORF1 polyprotein. Once induced, the MNV ORF1… Expand
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