Expression of the Helicobacter pylori ureI gene is required for acidic pH activation of cytoplasmic urease.

@article{Scott2000ExpressionOT,
  title={Expression of the Helicobacter pylori ureI gene is required for acidic pH activation of cytoplasmic urease.},
  author={David R Scott and Elizabeth A. Marcus and David L. Weeks and Adrian Susan Lee and Klaus Melchers and George Sachs},
  journal={Infection and immunity},
  year={2000},
  volume={68 2},
  pages={470-7}
}
ureI encodes an integral cytoplasmic membrane protein. It is present in the urease gene cluster of Helicobacter pylori and is essential for infection and acid survival, but its role is unknown. To determine the function of UreI protein, we produced H. pylori ureI deletion mutants and measured the pH dependence of urease activity of intact and lysed bacteria and the effect of urea on the membrane potential. We also determined ureI expression, urease activity, and the effect of urea on membrane… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 42 extracted citations

Pathogenesis of Helicobacter pylori infection.

Clinical microbiology reviews • 2006
View 5 Excerpts
Highly Influenced

References

Publications referenced by this paper.
Showing 1-10 of 28 references

Preparation of genomic DNA from bacteria.

Current protocols in molecular biology • 2001

Similar Papers

Loading similar papers…