Expression of the H-ras proto-oncogene is controlled by alternative splicing.

@article{Cohen1989ExpressionOT,
  title={Expression of the H-ras proto-oncogene is controlled by alternative splicing.},
  author={J. B. Cohen and Senka Broz and Arthur D. Levinson},
  journal={Cell},
  year={1989},
  volume={58 3},
  pages={461-72}
}
We previously demonstrated that a point mutation in the last intron of the human H-ras oncogene causes a significant increase in its expression and transforming efficiency. Here we establish the basis of this phenomenon. Using gene reconstruction experiments, we have identified a negative-acting element in the intron that is completely inactivated by the mutation. The effects of other nucleotide alterations introduced into this region suggested that the negative element might constitute an… CONTINUE READING