Expression of the Escherichia coli glutamate dehydrogenase gene in the cyanobacterium Synechococcus PCC6301 causes ammonium tolerance

@article{Lightfoot2004ExpressionOT,
  title={Expression of the Escherichia coli glutamate dehydrogenase gene in the cyanobacterium Synechococcus PCC6301 causes ammonium tolerance},
  author={David A. Lightfoot and Andrew J. Baron and John C. Wootton},
  journal={Plant Molecular Biology},
  year={2004},
  volume={11},
  pages={335-344}
}
The unicellular cyanobacterium Synechococcus PCC6301 lacks a hybridisable homologue of the strongly conserved gdhA gene of E. coli that encodes NADP-specific glutamate dehydrogenase. This is consistent with the failure to find this enzyme in extracts of the cyanobacterium. The E. coli gdhA gene was transferred to Synechococcus PCC6301 by transformation with an integrative vector. High levels of glutamate dehydrogenase activity, similar to those found in ammonium grown E. coli cells, were found… 
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References

SHOWING 1-10 OF 36 REFERENCES
The Klebsiella aerogenes glutamate dehydrogenase (gdhA) gene: cloning, high-level expression and hybrid enzyme formation in Escherichia coli
TLDR
The NADP-dependent glutamate dehydrogenase gene of Klebsiella aerogenes was cloned in E. coli in the expression plasmid pRK9 and the gdhA gene was determined and found to be strongly homologous with that of E.aerogenes.
Introduction of the Escherichia coli gdhA gene into Rhizobium phaseoli: effect on nitrogen fixation
TLDR
Rhizobium phaseoli cells that lost GDH activity and assimilated ammonium by the glutamine synthetase-glutamate synthase pathway preferentially nodulated Phaseolus vulgaris.
Ammonia assimilation in blue-green algae
TLDR
There was little difference in levels of GDH in cells growing with nitrate or with L-asparagine, while the levels of AlaDH were slightly lower in cells grown with L, asparagine; and GGAT was undetectable in N2-grown cells.
Identification of blue-green algal nitrogen fixation genes by using heterologous DNA hybridization probes.
TLDR
Cloned fragments of the Klebsiella pneumoniae nitrogen fixation (nif) genes are used as probes in DNA to identify and clone Anabaena nif genes, demonstrating the feasibility of using heterologous probes to identify genes for which no traditional genetic selection exists.
Transformation of the Cyanobacterium Synechococcus PCC 6301 Using Cloned DNA
TLDR
Stable ampicillin-resistant transformants of Synechococcus PCC 6301 were isolated using as donor fragments chromosomal DNA cloned into ColEl-derived vectors and transformation was achieved reproducibly at frequencies lower than those reported previously by other workers for the closely related strain PCC 7942.
Different promoters for the Anabaena glutamine synthetase gene during growth using molecular or fixed nitrogen
The major RNA products of the cyanobacterium (blue-green alga) Anabaena glutamine synthetase (glnA) gene are transcribed from different promoters during growth utilizing molecular or fixed nitrogen.
The incomplete tricarboxylic acid cycle in the blue-green alga Anabaena variabilis.
TLDR
The presence of an incomplete tricarboxylic acid cycle in Anabaena variabilis and Anacystis nidulans is described and the results are discussed in relation to possible reasons for autotrophic behaviour.
Characteristics of Heterotrophic Growth in the Blue-Green Alga Nostoc sp. Strain Mac
The blue-green alga Nostoc sp. strain Mac was grown heterotrophically in the dark on glucose and fructose. Cell composition was similar under photoautotrophic and heterotrophic growth conditions and
Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus
TLDR
The glutamate dehydrogenase gene of Escherichia coli has been cloned into broad host-range plasmids and can complement glutamate synthase mutants of Methylophilus methylotrophus, thus the recombinant organism converts more growth substrate, methanol, into cellular carbon.
...
...