Expression of the E.coli 3-methyladenine DNA glycosylase I gene in mammalian cells reduces the toxic and mutagenic effects of methylating agents.

@article{Klungland1992ExpressionOT,
  title={Expression of the E.coli 3-methyladenine DNA glycosylase I gene in mammalian cells reduces the toxic and mutagenic effects of methylating agents.},
  author={Arne Klungland and L. J. Fairbairn and Alastair JM Watson and G. P. Margison and Erling Seeberg},
  journal={The EMBO journal},
  year={1992},
  volume={11 12},
  pages={4439-44}
}
In order to investigate the importance of 3-methyladenine in cellular sensitivity to chemical methylating agents we have constructed retroviral vectors for the integration and expression of the Escherichia coli tag gene in mammalian cells. The tag gene encodes 3-methyladenine DNA glycosylase-1 which specifically removes 3-alkyladenines from DNA. The constructs were introduced into Chinese hamster V79 cells by liposome mediated transfection or into murine haemopoietic stem cells by cocultivation… CONTINUE READING