Expression of the Alkaline Pectate Lyase Gene pel from Paenibacillus campinasensis BL-11 in Pichia pastoris

@inproceedings{Xiaoxia2015ExpressionOT,
  title={Expression of the Alkaline Pectate Lyase Gene pel from Paenibacillus campinasensis BL-11 in Pichia pastoris},
  author={Liu Xiao-xia},
  year={2015}
}
The alkaline pectate lyase gene pel from Paenibacillus campinasensis BL-11 was synthesized by total gene synthesis, cloned on a p HKA carrier, and actively expressed in Pichia pastoris GS115. The signal peptide and promoter were optimized, so that the pectate lyase activity reached 432.36 U/m L after induction was carried out by 168-h shake flask fermentation. The basic enzymatic properties of the recombinant pectate lyase were preliminarily measured, and the optimal p H and temperature were 10… CONTINUE READING