Expression of the ACC Deaminase Gene fromEnterobacter cloacae UW4 in Azospirillum brasilense

  title={Expression of the ACC Deaminase Gene fromEnterobacter cloacae UW4 in Azospirillum brasilense},
  author={G. J. E. Holguin and Bernard R. Glick},
  journal={Microbial Ecology},
The ACC deaminase structural gene (acdS) from Enterobacter cloacae UW4 was cloned in the broad host range plasmid pRK415 under the control of the lac promoter and transferred into Azospirillum brasilense Cd and Sp245. A. brasilenseCd and Sp245 transformants showed high ACC deaminase activity, similar to that observed in Enterobacter cloacae UW4. The expression of ACC deaminase improved the existing growth promoting activity of Azospirillum. The roots of tomato and canola seedlings were… CONTINUE READING

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