Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons.

@article{Makoff1989ExpressionOT,
  title={Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons.},
  author={Andrew J. Makoff and M. D. Oxer and Michael A. Romanos and Neil F Fairweather and Stuart P. Ballantine},
  journal={Nucleic acids research},
  year={1989},
  volume={17 24},
  pages={10191-202}
}
Tetanus toxin fragment C had been previously expressed in Escherichia coli at 3-4% cell protein. The codon bias for tetanus toxin in Clostridium tetani is very different from that of highly expressed homologous genes in E. coli, resulting in the presence of many rare E. coli codons in the sequence encoding fragment C. We have replaced the coding sequence by sequence optimized for codon usage in E. coli, and show that the expression of fragment C is increased. Although the level of mRNA also… CONTINUE READING

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