• Biology
  • Published 2012

Expression of steroidogenic enzyme in subclinical Cushing's syndrome due to adrenal adenomas

@inproceedings{Xinsheng2012ExpressionOS,
  title={Expression of steroidogenic enzyme in subclinical Cushing's syndrome due to adrenal adenomas},
  author={Wang Xin-sheng},
  year={2012}
}
Objective To determine whether steroidogenic enzyme expression is associated with the steroid secretory pattern of subclinical Cushing's syndrome(SCS) by investigating the expression of CYP17 and CYP11B1 in SCS adenomas,normal adrenal cortices(NA),non-functional adrenal adenomas(NFA) and cortisol-producing adenomas(CPA).Methods Total RNA and protein were extracted from 20 CPA,6 SCS,15 NFA,and 8 NA.Real-time quantitative polymerase chain reaction(PCR),western blotting and immunohistochemistry… CONTINUE READING