Expression of soluble recombinant lipoxygenase from Pleurotus sapidus in Pichia pastoris.

@article{Kelle2014ExpressionOS,
  title={Expression of soluble recombinant lipoxygenase from Pleurotus sapidus in Pichia pastoris.},
  author={Sebastian Kelle and Kateryna Zelena and Ulrich Krings and Diana Linke and Ralf Guenter Berger},
  journal={Protein expression and purification},
  year={2014},
  volume={95},
  pages={233-9}
}
The first heterologous expression of an iron-containing lipoxygenase from a basidiomycete in Pichia pastoris is reported. Five different expression constructs of the lipoxygenase gene LOX1 from Pleurotus sapidus were cloned and successfully transferred into P. pastoris SMD1168, but only one pPIC9K vector construct was functionally expressed. In this… CONTINUE READING