Expression of soluble guanylyl cyclase. Catalytic activity requires two enzyme subunits.

@article{Harteneck1990ExpressionOS,
  title={Expression of soluble guanylyl cyclase. Catalytic activity requires two enzyme subunits.},
  author={Christian Harteneck and Doris Koesling and Ariane S{\"o}ling and Guenter Schultz and Eycke B{\"o}hme},
  journal={FEBS letters},
  year={1990},
  volume={272 1-2},
  pages={
          221-3
        }
}
Purified soluble guanylyl cyclase consists of two subunits (70 and 73 kDa) whose primary structures were recently determined. The availability of cDNA clones coding for either subunit allowed to study the question of the functional roles of the two subunits in expression experiments. Enzyme subunits were expressed in COS-7 cells by transfection with expression vectors containing the coding region for the 70 of 73 kDa subunit of the enzyme. No significant elevation in the activity of soluble… CONTINUE READING

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