Expression of simian virus 40 large T (tumor) oncogene in mouse chondrocytes induces cell proliferation without loss of the differentiated phenotype.

@article{MallinGrin1993ExpressionOS,
  title={Expression of simian virus 40 large T (tumor) oncogene in mouse chondrocytes induces cell proliferation without loss of the differentiated phenotype.},
  author={Fr{\'e}d{\'e}ric Mall{\'e}in-G{\'e}rin and Bjorn Reino Olsen},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90 8},
  pages={3289-93}
}
We have infected primary embryonic mouse limb chondrocytes with a retrovirus carrying simian virus 40 early regions and have obtained a monoclonal mouse chondrocyte line, MC615, that was able to grow on culture dishes for at least 7 months and 20 passages. MC615 cells show expression of simian virus 40 large T (tumor) antigen and express markers characteristic of cartilage in vivo, such as types II, IX, and XI collagen, as well as cartilage aggrecan and link protein. These data show that cell… CONTINUE READING