Expression of rice (Oryza sativa L. var. Nipponbare) alpha-galactosidase genes in Escherichia coli and characterization.

@article{Li2007ExpressionOR,
  title={Expression of rice (Oryza sativa L. var. Nipponbare) alpha-galactosidase genes in Escherichia coli and characterization.},
  author={Suhong Li and Wook-Dong Kim and Satoshi Kaneko and Prukutty A Prema and Mitsutoshi Nakajima and Hideyuki Kobayashi},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2007},
  volume={71 2},
  pages={520-6}
}
Two putative alpha-galactosidase genes from rice (Oryza sativa L. var. Nipponbare) belonging to glycoside hydrolase family 27 were cloned and expressed in Escherichia coli. These enzymes showed alpha-galactosidase activity and were purified by Ni Sepharose column chromatography. Two purified recombinant alpha-galactosidases (alpha-galactosidase II and III… CONTINUE READING