Expression of ribonucleolytic toxin restrictocin in Escherichia coli: purification and characterization.

@article{Rathore1996ExpressionOR,
  title={Expression of ribonucleolytic toxin restrictocin in Escherichia coli: purification and characterization.},
  author={Dharmendar Rathore and Saroj K Nayak and Janendra K. Batra},
  journal={FEBS letters},
  year={1996},
  volume={392 3},
  pages={259-62}
}
Restrictocin is a toxin produced by the fungus Aspergillus restritus. The DNA coding for restrictocin was isolated from the host by polymerase chain reaction and cloned into a T7 promoter-based expression vector. The protein was overproduced in Escherichia coli and remained insoluble in the cell in the form of inclusion bodies. Recombinant restrictocin was… CONTINUE READING