Expression of recombinant tilapia insulin-like growth factor-I and stimulation of juvenile tilapia growth by injection of recombinant IGFs polypeptides

@inproceedings{Chen1999ExpressionOR,
  title={Expression of recombinant tilapia insulin-like growth factor-I and stimulation of juvenile tilapia growth by injection of recombinant IGFs polypeptides},
  author={Jyh-Yih Chen and Jian-Chyi Chen and Chi-Yao Chang and Shih-Chieh Shen and M Chen and Jen-Leih Wu},
  year={1999}
}
Ž . Mature recombinant tilapia insulin-like growth factor-I IGF-I polypeptide was produced in Ž Escherichia coli by cloning the IGF-I B to D domains with glutathione-S-transferase GST, . pGEX-2T vector . The recombinant IGF-I fusion protein, produced following induction of E. coli with IPTG induction and digestion with thrombin, appeared as a major protein band with a Žw x . molecular mass of 7 kDa. Recombinant tilapia IGF-I GSPGIHM -IGF-I polypeptide bioactivity, w x as measured in a… CONTINUE READING