Expression of recombinant proteins in a lipid A mutant of Escherichia coli BL21 with a strongly reduced capacity to induce dendritic cell activation and maturation.

@article{Cognet2003ExpressionOR,
  title={Expression of recombinant proteins in a lipid A mutant of Escherichia coli BL21 with a strongly reduced capacity to induce dendritic cell activation and maturation.},
  author={Isabelle Cognet and Am{\'e}lie Benoit de Coignac and Giovanni Magistrelli and Pascale Jeannin and J C Aubry and Karine Maisnier-Patin and Gersende Caron and Sylvie Chevalier and Fr{\'e}d{\'e}ric Humbert and Thien Nga Nguyen and Alain Beck and Dominique Velin and Yves Delneste and Martine Malissard and Jean-François Gauchat},
  journal={Journal of immunological methods},
  year={2003},
  volume={272 1-2},
  pages={199-210}
}
Mutations in the Escherichia coli (E. coli) and Salmonella lpxM gene have been shown to result in strains which grow normally and which produce a non-myristoylated lipopolysaccharide (nmLPS) with strongly reduced endotoxicity. Using homologous recombination, we inactivated the lpxM gene in BL21 (DE3), a strain widely used for the production of recombinant proteins. This led to a derivative unaffected in its capacity to support the production of recombinant proteins. This new strain expresses… CONTINUE READING
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