Expression of recombinant human acid sphingomyelinase in insect Sf21 cells: purification, processing and enzymatic characterization.

@article{Bartelsen1998ExpressionOR,
  title={Expression of recombinant human acid sphingomyelinase in insect Sf21 cells: purification, processing and enzymatic characterization.},
  author={Oliver Bartelsen and Stephanie Lansmann and Michael Nettersheim and Thorsten Lemm and Klaus Ferlinz and Konrad Sandhoff},
  journal={Journal of biotechnology},
  year={1998},
  volume={63 1},
  pages={29-40}
}
Biochemical and structural studies on human acid sphingomyelinase (haSMase) depend on the access to homogeneous biologically active enzyme. Due to the low abundance of native haSMase (n-haSMase) in human tissue, conventional purification strategies are not suitable for the isolation of preparative amounts of the enzyme. We describe a novel approach to the functional expression and purification of haSMase employing the baculovirus expression vector system. Infection of Spodoptera frugiperda 21… CONTINUE READING
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