Expression of rat alpha 1 Na,K-ATPase containing substitutions of "essential" amino acids in the catalytic center.

@article{Lane1993ExpressionOR,
  title={Expression of rat alpha 1 Na,K-ATPase containing substitutions of "essential" amino acids in the catalytic center.},
  author={Lois K. Lane and Jennifer M Feldmann and C E Flarsheim and C L Rybczynski},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 24},
  pages={17930-4}
}
Previous chemical modification studies have suggested that Cys-369, Cys-658, Lys-482, Asp-712, and Asp-716 are essential for Na,K-ATPase function. To determine if the side chains of these amino acid residues are required for enzyme activity, rat alpha 1 cDNAs containing the mutations Cys-369-->Ser, Cys-658-->Ala, Lys-482-->Ala, Asp-712-->Asn, and Asp-716-->Asn were prepared and stably expressed in HeLa cells, which normally cannot survive in medium containing microM concentrations of ouabain… CONTINUE READING