Expression of rabbit ileal N3 Na+/nucleoside cotransport activity in Xenopus laevis oocytes.

@article{Redlak1996ExpressionOR,
  title={Expression of rabbit ileal N3 Na+/nucleoside cotransport activity in Xenopus laevis oocytes.},
  author={Maria J. Redlak and Zendra E. Zehner and Scott Betcher},
  journal={Biochemical and biophysical research communications},
  year={1996},
  volume={225 1},
  pages={
          106-11
        }
}
To determine if the Na+/nucleoside cotransport activity in the distal rabbit intestine has either purine-selective (N1) or broad (N3) substrate specificity, Na(+)-dependent inosine uptake was expressed in Xenopus laevis oocytes. The rate of expressed Na(+)-stimulated inosine uptake saturated with increasing inosine concentration (apparent K(m) = 58.2 +/- 13.0 microM), was insensitive to inhibition by 6-(4-nitrobenzyl)thio-9-beta-D-ribofuranosylpurine, and was partially inhibited by phloridzin… CONTINUE READING