Expression of protein kinase C isoforms in HL60 and phorbol ester resistant HL525 cells.

Isoform protein expression and activity were investigated to evaluate whether differential expression of protein kinase C (PKC) accounts for the inability of HL525 cells to respond to phorbol 12-13,myristate acetate (PMA). Immunoblotting analysis of lysates from both the parental HL60 and PMA-resistant HL525 cells revealed expression of alpha-, beta-, delta… CONTINUE READING