Expression of prostate-specific membrane antigen (PSMA) after the transfection of shRNA lentivirus with PSMA construction in 293 cells

@inproceedings{Jian2008ExpressionOP,
  title={Expression of prostate-specific membrane antigen (PSMA) after the transfection of shRNA lentivirus with PSMA construction in 293 cells},
  author={Huang Jian},
  year={2008}
}
AIM:To obtain shRNA sequences that can stably block the expression of prostate-specific membrane antigen(PSMA)in the prostate cancer cell line LNCaP and construct the lentivirus vector.METHODS:According to genetic information,we design siRNA1,siRNA2 and siRNA3 of PSMA,the three siRNA sequences targeting the cds area of PSMA gene and then forming the corresponding four pairs of complementary single strand DNA of shRNA,including the sense strand and the antisense strand.The sequence of sense… CONTINUE READING