Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L., and its purification and characterization

@article{Wang2005ExpressionOP,
  title={Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L., and its purification and characterization},
  author={Yizhen Wang and Xiao-feng Wu and Guangfu Liu and Cuiping Cao and Haiqing Huang and Zirong Xu and Jianxin Liu},
  journal={Applied Microbiology and Biotechnology},
  year={2005},
  volume={69},
  pages={385-389}
}
Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 μg of rPLF was purified from a single silkworm pupa infected by the virus and the rPLF was proved to be biologically active. This method established in our study will pave the way for efficient industrial production… CONTINUE READING