Expression of polyhedrin-hEGF fusion protein in cultured cells and larvae of Bombyx mori

Abstract

For mass production of human epidermal growth factor (hEGF), silkworm baculovirus expression vector system (BEVS) was adopted in this study. hEGF gene was in-frame fused with polyhedrin (Ph) gene under the control of Ph promoter and was used to co-transfect BmN cell with the modified Bombyx mori baculovirus DNA to obtained recombinant virus. The ELISA… (More)

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