Expression of penicillin G acylase from the cloned pac gene of Escherichia coli ATCC11105. Effects of pacR and temperature.

@article{Dai2001ExpressionOP,
  title={Expression of penicillin G acylase from the cloned pac gene of Escherichia coli ATCC11105. Effects of pacR and temperature.},
  author={Ming-hua Dai and Yusheng Zhu and Y L Yang and En Duo Wang and Yuqing Xie and Guo-Ping Zhao and Weihua Jiang},
  journal={European journal of biochemistry},
  year={2001},
  volume={268 5},
  pages={
          1298-303
        }
}
The structural gene pac in Eschericia coli ATCC11105 encodes penicillin G acylase (PGA). Within the pac gene, there is a regulatory gene pacR, which is transcribed in the opposite direction. Site-directed mutagenesis was performed at base 1045 of pac by replacing a T with a C. This substitution did not alter the amino-acid sequence of PGA, but changed the translation start codon of pacR from AUG to GUG. The expression of the mutant pacR decreased dramatically and the lacZ transcriptional fusion… CONTINUE READING

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