Expression of osmotic stress-related genes in tissues of normal and hyposmotic rats.

@article{Zhang2003ExpressionOO,
  title={Expression of osmotic stress-related genes in tissues of normal and hyposmotic rats.},
  author={Zheng Zhang and Joan D. Ferraris and Heddwen L. Brooks and Ioana Brisc and Maurice B. Burg},
  journal={American journal of physiology. Renal physiology},
  year={2003},
  volume={285 4},
  pages={F688-93}
}
TonEBP is a transcription factor that, when activated by hypertonicity, increases transcription of genes, including those involved in organic osmolyte accumulation. Surprisingly, it is expressed in virtually all tissues, including many never normally exposed to hypertonicity. We measured TonEBP mRNA (real-time PCR) and protein (Western blot analysis) in tissues of control (plasma osmolality 294 +/- 1 mosmol/kgH2O) and hyposmotic (dDAVP infusion plus water loading for 3 days, 241 +/- 2 mosmol… CONTINUE READING