• Corpus ID: 17459547

Expression of olfactory receptors during development in Xenopus laevis.

@article{Mezler1999ExpressionOO,
  title={Expression of olfactory receptors during development in Xenopus laevis.},
  author={Mario Mezler and S Konzelmann and Joachim Freitag and Patricia Roessler and Heinz Breer},
  journal={The Journal of experimental biology},
  year={1999},
  volume={202 Pt 4},
  pages={
          365-76
        }
}
A coordinated expression of tissue- and cell-specific genes during development is required to establish the complex functional organization of the vertebrate olfactory system. Owing to the unique features of its olfactory system and the well-characterized phases of its development, Xenopus laevis was chosen as a model organism to study the onset and the temporal and spatial patterns of expression of olfactory-specific genes. Using RT-PCR and in situ hybridization, it was found that expression… 

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References

SHOWING 1-10 OF 69 REFERENCES
Expression of olfactory receptor and transduction genes during rat development.
Ontogeny of odorant receptor gene expression in zebrafish, Danio rerio.
We cloned three putative odorant receptor (OR) genes from the zebrafish to use as in situ hybridization probes to follow the temporal patterns of neurons expressing OR genes through a developmental
Cellular and molecular interactions in the development of the Xenopus olfactory system.
TLDR
Forebrain development is dependent on the presence of the placode and subsequent innervation by olfactory axons, with the extent of this dependence declining as development advances, as well as downstream genes expressed in the anterior neural ridge and sense plate.
Receptor Expression in Olfactory Neurons During Rat Development: In Situ Hybridization Studies
TLDR
The critical phase for the maturation of the olfactory system from embryonic day (E) 12 to E18 was analysed and spatial segregation of receptor subtypes in distinct expression zones was observed.
Spatial pattern of receptor expression in the olfactory epithelium.
TLDR
A PCR-based strategy for amplifying putative receptors involved in murine olfaction was employed to isolate a member (OR3) of the seven-transmembrane-domain receptor superfamily that may serve a functional role in odor coding or, alternatively, may play a role in the development of the olfactory system.
Spatial patterns of olfactory neurons expressing specific odor receptor genes in 48-hour-old embryos of zebrafish Danio rerio.
TLDR
The spatial patterns of olfactory neurons expressing specific OR genes in 48 h embryos are examined, consistent with those observed from adult tissue and support models suggesting that extrinsic positional cues do not have a major role in specifying Olfactory neuron phenotypes.
Two olfactory marker proteins in Xenopus laevis
TLDR
Two homologous genes encoding olfactory marker proteins have been identified that share a sequence identity with mammalian OMPs of about 50% in Xenopus laevis; sequence comparison revealed significant variability in the N‐terminus and C-terminus regions; in contrast, two internal domains were highly conserved between amphibian and mammalian O MPs, suggesting some functional relevance.
Neurogenesis in the olfactory bulb of the frogXenopus Laevis shows unique patterns during embryonic development and metamorphosis
...
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