Expression of nitric oxide synthase in the preoptic-hypothalamo-hypophyseal system of the teleost Oreochromis niloticus.

@article{Bordieri2003ExpressionON,
  title={Expression of nitric oxide synthase in the preoptic-hypothalamo-hypophyseal system of the teleost Oreochromis niloticus.},
  author={Loredana Bordieri and Tiziana Persichini and Giorgio Venturini and Carla Cioni},
  journal={Brain, behavior and evolution},
  year={2003},
  volume={62 1},
  pages={43-55}
}
In the present study, we have analyzed the expression of nitric oxide synthase (NOS) in the preoptic-hypothalamo-hypophyseal system of the teleost Oreochromis niloticus. The assay for enzyme activity demonstrated that a constitutive NOS activity is present both in soluble and particulate fractions of the homogenates of diencephalons. Western blot analysis using an antibody against the N-terminus of human nNOS revealed two bands both in the supernatant and in the pellet. One band co-migrates at… CONTINUE READING

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