Expression of nifH genes in natural microbial assemblages in Lake George, New York, detected by reverse transcriptase PCR.

@article{Zani2000ExpressionON,
  title={Expression of nifH genes in natural microbial assemblages in Lake George, New York, detected by reverse transcriptase PCR.},
  author={Sabino Zani and Mark Mellon and Jackie L Collier and Jonathan P. Zehr},
  journal={Applied and environmental microbiology},
  year={2000},
  volume={66 7},
  pages={
          3119-24
        }
}
A modified nested reverse transcriptase PCR (RT-PCR) method was used to detect the expression of nitrogenase genes in meso-oligotrophic Lake George, New York. Net (>20-microm pore size) plankton samples collected from two sites (Dome Island and Hague Marina) were extracted for total RNA and genomic DNA to determine the identity of diazotrophic organisms that were present and those that were actively expressing nitrogenase genes. Phylogenetic analysis of individual sequences cloned from PCR… CONTINUE READING
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