Expression of neuronal markers during NTera2/cloneD1 differentiation by cell aggregation method

@article{Megiorni2005ExpressionON,
  title={Expression of neuronal markers during NTera2/cloneD1 differentiation by cell aggregation method},
  author={Francesca Megiorni and B{\'a}rbara Mora and Paola Indovina and Maria Cristina Mazzilli},
  journal={Neuroscience Letters},
  year={2005},
  volume={373},
  pages={105-109}
}
Human teratocarcinoma NTera2/cloneD1 (NT2) cells are able to generate postmitotic neurons in response to retinoic acid (RA) and for this reason these cells provide an important tool to study human neurogenesis in vitro. We have obtained neurons by treating NT2 aggregated cells with RA for solely 14 days. RT-PCR assays showed that NT2 cells express mRNAs of several neural bHLH genes such as Hes1, Ngn1, Mash1, NeuroD, Math1 and Pax6, just in the early days of RA exposure. In particular, we… CONTINUE READING
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