Expression of multiple CPB genes encoding cysteine proteases is required for Leishmania mexicana virulence in vivo.

@article{Denise2003ExpressionOM,
  title={Expression of multiple CPB genes encoding cysteine proteases is required for Leishmania mexicana virulence in vivo.},
  author={Hubert Denise and Kathryn S McNeil and Darren R Brooks and J. -P. Alexander and Graham H. Coombs and Jeremy C Mottram},
  journal={Infection and immunity},
  year={2003},
  volume={71 6},
  pages={3190-5}
}
Leishmania mexicana mutants deficient in the multicopy CPB gene array have reduced virulence, demonstrated by poor lesion growth in BALB/c mice and induction of a protective Th1 response. Reinsertion of the amastigote-specific CPB2.8 or metacyclic stage-specific CPB2 gene into a CPB-deficient mutant L. mexicana failed to restore either a Th2 response or sustained virulence. However, reexpression of multiple CPB genes from a cosmid significantly restored virulence. This was characterized by… CONTINUE READING

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