Expression of metallothionein genes I and II in bank vole Clethrionomys glareolus populations chronically exposed in situ to heavy metals.

@article{wiergoszKowalewska2007ExpressionOM,
  title={Expression of metallothionein genes I and II in bank vole Clethrionomys glareolus populations chronically exposed in situ to heavy metals.},
  author={Renata Świergosz-Kowalewska and Agnieszka Bednarska and Amanda Callaghan},
  journal={Environmental science & technology},
  year={2007},
  volume={41 3},
  pages={1032-7}
}
The expression of two metallothionein genes (Mt-I and Mt-II) in the liver, kidney, and gonad of bank voles collected at four metal-contaminated sites (Cd, Zn, Pb, and Fe) were measured using the quantitative real-time PCR method (QPCR). Relative Mt gene expression was calculated by applying a normalization factor (NF) using the expression of two housekeeping genes, ribosomal 18S and beta-actin. Relative Mt expression in tissues of animals from contaminated sites was up to 54.8-fold higher than… CONTINUE READING