Expression of memory to the terminal differentiation inducing activity of tiazofurin in HL-60 leukemia cells.

  title={Expression of memory to the terminal differentiation inducing activity of tiazofurin in HL-60 leukemia cells.},
  author={John A. Sokoloski and Alan C. Sartorelli},
  journal={Leukemia research},
  volume={15 5},
Tiazofurin, a potent inhibitor of inosine 5'-phosphate dehydrogenase, depletes guanine nucleotide pools and induces granulocytic maturation of HL-60 leukemia cells. These effects are reversed when cells exposed to this agent for 24 h are washed and placed in tiazofurin-free medium. HL-60 cells treated with tiazofurin for a 24 h period, retain a precommitment memory that lessens the time interval necessary for cells to express the mature phenotype upon re-exposure. That protein synthesis was… Expand
2 Citations
Synergistic growth inhibitory and differentiating effects of trimidox and tiazofurin in human promyelocytic leukemia HL-60 cells.
The results suggest that trimidox in combination with tiazofurin might be useful in the treatment of leukemia. Expand
3,5,3'-Triiodothyronine stimulates retinoic acid-induced differentiation in HL-60 cells
The present finding that T 3 potentiates RA-induced HL-60 cell differentiation may raise the possibility that T3 supplement increases clinical remission in APL patients who are treated with RA. Expand


Synergistic action of tiazofurin and retinoic acid on differentiation and colony formation of HL-60 leukemia cells.
Tiazofurin and retinoic acid synergistically induced differentiation and inhibited colony formation in HL-60 human promyelocytic leukemia cells in cell culture. The synergism was the result ofExpand
Memory of MEL cells to a previous exposure to inducer
Results suggest that a cellular component necessary for the commitment event accumulates in response to inducer and that this component has a decay time on the order of 10 hr. Expand
Alterations in glycoprotein synthesis and guanosine triphosphate levels associated with the differentiation of HL-60 leukemia cells produced by inhibitors of inosine 5'-phosphate dehydrogenase.
The exposure of HL-60 leukemia cells to inhibitors of IMP dehydrogenase caused a marked reduction in the incorporation of [3H]mannose into both cellular glycoproteins and their lipid-linked oligosaccharide precursors; these effects are presumably due to the pronounced decrease in intracellular levels of guanosine triphosphate produced by blockage of IMP dehydration. Expand
Structure-activity relationships for the induction of differentiation of HL-60 human acute promyelocytic leukemia cells by anthracyclines.
The results suggest that certain anthracyclines would be reasonable candidate drugs to use in a clinical trial aimed at reducing the leukemic stem cell burden through maturation rather than through cytodestruction. Expand
Tiazofurin action in leukemia: evidence for down-regulation of oncogenes and synergism with retinoic acid.
Because both tiazofurin and retinoic acid are licensed drugs, their potential use in combination chemotherapy may have clinical relevance in the treatment of end-stage leukemia where earlier studies have demonstrated the usefulness of tiaz ofurin. Expand
Tiazofurin: biological effects and clinical uses.
Better patient selection, limitation of treatment duration and earlier recognition and treatment of complications have now made it possible to administer tiazofurin without undue toxicity, and a good correlation between biochemical parameters and clinical response was demonstrated in leukemic patients. Expand
Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid.
This study suggests that retinoids could provide a therapeutic tool in the treatment of acute myeloid leukemia, and indicates thatretinoids, in addition to their well-characterized involvement in epithelial cell differentiation, may also be involved in the differentiation of certain hematopoietic cells. Expand
Biochemically directed therapy of leukemia with tiazofurin, a selective blocker of inosine 5'-phosphate dehydrogenase activity.
Tiazofurin was better tolerated in most patients than other antileukemic treatment modalities and provided a rational,Biochemically targeted, and biochemically monitored chemotherapy which should be of interest in the treatment of leukemias and as a paradigm in enzyme pattern-targeted chemotherapy. Expand
Control of HL-60 myeloid differentiation. Evidence of uncoupled growth and differentiation control, S-phase specificity, and two-step regulation.
  • A. Yen
  • Biology, Medicine
  • Experimental cell research
  • 1985
The present results for DSMO showed that the two inducers effect different cellular pathways for differentiation of HL-60 cells to mature myeloid cells, but with certain common features including the above S-phase specificity and pre-commitment memory. Expand
Induction of differentiation of human promyelocytic leukemia cells (HL-60) by nucleosides and methotrexate.
Various purine and pyrimidine analogs and methotrexate were tested to determine whether they induce morphologic and functional myeloid differentiation in HL-60, a human promyelocytic leukemia cellExpand