Expression of melanin-related genes in cultured adult human retinal pigment epithelium and uveal melanoma cells.

@article{Lu2007ExpressionOM,
  title={Expression of melanin-related genes in cultured adult human retinal pigment epithelium and uveal melanoma cells.},
  author={F. -J. Lu and Dongsheng Yan and Xiangtian Zhou and Dan-Ning Hu and Jia Qu},
  journal={Molecular vision},
  year={2007},
  volume={13},
  pages={2066-72}
}
PURPOSE Controversy exists over melanogenesis of adult human RPE cells in vitro. This study investigated melanin content and production and expression of tyrosinase (TYR), tyrosinase-related-protein-1 (TRP1), tyrosinase-related-protein-2 (TRP2), and P gene in cultured human RPE cells and uveal melanoma cells. METHODS RPE cells were isolated and cultured from three adult donor eyes. A continuous human uveal melanoma cell line was established from primary choroidal melanoma. Melanin content and… CONTINUE READING