Expression of laccase IIIb from the white-rot fungus Trametes versicolor in the yeast Yarrowia lipolytica for environmental applications

@article{Jolivalt2004ExpressionOL,
  title={Expression of laccase IIIb from the white-rot fungus Trametes versicolor in the yeast Yarrowia lipolytica for environmental applications},
  author={Claude Jolivalt and Catherine Madzak and Agathe Brault and Eliane Caminade and Christian Malosse and Christian Mougin},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={66},
  pages={450-456}
}
Improvement of the catalytic properties of fungal laccases is a current challenge for the efficient bioremediation of natural media polluted by xenobiotics. We developed the heterologous expression of a laccase from the white-rot fungus Trametes versicolor in the yeast Yarrowia lipolytica as a first step for enzyme evolution. The full-length cDNA consisted of a 1,561-bp open reading frame encoding lacIIIb, a 499-amino-acid protein and a 21-amino-acid signal peptide. Native and yeast secretion… CONTINUE READING

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