Expression of ksdD gene encoding 3-ketosteroid-Delta1-dehydrogenase from Arthrobacter simplex in Bacillus subtilis.

@article{Li2007ExpressionOK,
  title={Expression of ksdD gene encoding 3-ketosteroid-Delta1-dehydrogenase from Arthrobacter simplex in Bacillus subtilis.},
  author={Yingxia Li and Fuping Lu and Tao Sun and L Du},
  journal={Letters in applied microbiology},
  year={2007},
  volume={44 5},
  pages={563-8}
}
AIMS To improve KSDH enzyme activity and the transformation level for androst-4-ene-3,17-dione. METHODS AND RESULTS 3-ketosteroid-Delta(1)-dehydrogenase gene from Arthrobacter simplex was expressed in Bacillus subtilis under the control of P43 promoter. The molecular weight of expressed enzyme was about 55 kDa by SDS-PAGE analysis. The activities of intracellular and extracellular soluble enzymes examined by spectrophotometrical method were 110 +/- 0.5 mU mg(-1) and 15 +/- 0.6 mU mg(-1) of… CONTINUE READING

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