Expression of jumper ant (Myrmecia pilosula) venom allergens: Post‐translational processing of allergen gene products

  title={Expression of jumper ant (Myrmecia pilosula) venom allergens: Post‐translational processing of allergen gene products},
  author={G. R. Donovan and Mark D. Street and Tim Tetaz and A. Ian Smith and Dianne Alewood and Paul F. Alewood and S. K. Sutherland and Brian A Baldo},
  journal={IUBMB Life},
N‐terminal analyses of electrophoretically‐separated allergenic polypeptides of the venom of the jumper ant M. pilosula showed that five out of the six allergenic polypeptides identified are homologous with the cloned major allergen Myr p I and may be derived from a single precursor polypeptide. The sixth polypeptide is homologous with a second cloned major allergen, Myr p II which is expressed as a single precursor polypeptide but exists in its native form as a disulphide bond‐linked complex. 

Proteomic analysis of Myrmecia pilosula (jack jumper) ant venom.

Original article: Myrmecia pilosula (Jack Jumper) ant venom: identification of allergens and revised nomenclature

The aims were to determine the allergenicity of previously described venom peptides in their native forms, identify additional allergens and if necessary, update nomenclature used to describe the allergens according to International Union of Immunological Societies criteria.

Characterisation of major peptides in 'jack jumper' ant venom by mass spectrometry.

Stability of Myrmecia pilosula (Jack Jumper) Ant venom for use in immunotherapy.

Flow cytometric analysis of cell killing by the jumper ant venom peptide pilosulin 1.

Flow cytometry was used to investigate the cytotoxicity of the peptide for human white blood cells and found that pilosulin 1 killed cells within minutes, with kinetics similar to those of melittin, which was a slightly more potent cytotoxic agent thanmelittin.

Thymoquinone ameliorates Pachycondyla sennaarensis venom-induced acute toxic shock in male rats

Nigella sativa derived plant extract component, thymoquinone (TQ) has been tested against the Samsun ant venom (SAV) and effectively restores many of the biochemical and oxidative stress parameters comparable to the normal concomitant with improving the immunological aspects that might attributive in relieving from SAV-induced toxicity and allergic reactions in the affected organism to a greater extent.

Discovery of Defense- and Neuropeptides in Social Ants by Genome-Mining

A genome-mining approach was devised to identify defense- and neuropeptides in the genomes of social ants from Atta cephalotes (leaf-cutter ant), Camponotus floridanus (carpenter ant) and Harpegnathos saltator (basal genus), and annotated genes that encode oxytocin/vasopressin-related peptides (inotocins) and their putative receptors.

Insect molecular biology: an Australian perspective

There is minimal collaboration between insect molecular biologists and researchers working in traditional or comparative molecular biology in Australia, and it is proposed that an increase in these types of collaborations would benefit the broad field of entomology in Australia and increase the impact of AustralianEntomology globally.