Expression of human thyroid peroxidase in insect cells using recombinant baculovirus

@article{Kendler1993ExpressionOH,
  title={Expression of human thyroid peroxidase in insect cells using recombinant baculovirus},
  author={David L. Kendler and Veronica M. Brennan and Terry F Davies and Ronald P. Magnusson},
  journal={Molecular and Cellular Endocrinology},
  year={1993},
  volume={93},
  pages={199-206}
}
The baculovirus expression system was used to overexpress recombinant human thyroid peroxidase. Sf-9 cells infected with the recombinant virus AcMNPV-hTPO synthesized hTPO protein (hTPO-bac) immunogenic on Western blots when probed with either rabbit anti-TPO peptide sera or pooled human anti-TPO sera (MS12/89). hTPO-bac was a major constituent of the membrane fraction from the infected cells, constituting 14.9% and 10.1% of the 1% deoxycholate-soluble and insoluble fractions, respectively, as… CONTINUE READING
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