Expression of human parathyroid hormone in mammalian cells, Escherichia coli and Saccharomyces cerevisiae.

@article{Rokkones1994ExpressionOH,
  title={Expression of human parathyroid hormone in mammalian cells, Escherichia coli and Saccharomyces cerevisiae.},
  author={Erik Rokkones and B N Kareem and Ole Kristoffer Olstad and A H\ogset and K Schenstr\om and Lennart Hansson and Kaare M. Gautvik},
  journal={Journal of biotechnology},
  year={1994},
  volume={33 3},
  pages={293-306}
}
The entire human parathyroid hormone (hPTH) cDNA gene with its natural signal and pro-region is expressed in transfected mouse mammary tumor cells (C127I cells) and Chinese hamster lung cells (DON cells) under control of the murine metallothioneine-1 promoter in a vector in which replication functions are provided by the entire genome of bovine papilloma virus type I (BPV-1). Authentic hPTH is efficiently produced by the non-endocrine cells and secreted to the growth medium without any abberant… CONTINUE READING