Expression of human butyrylcholinesterase with an engineered glycosylation profile resembling the plasma-derived orthologue.

@article{Schneider2014ExpressionOH,
  title={Expression of human butyrylcholinesterase with an engineered glycosylation profile resembling the plasma-derived orthologue.},
  author={Jeannine D. Schneider and Alexandra Castilho and Laura J. Neumann and Friedrich Altmann and Andreas Loos and Latha Kannan and Tsafrir S Mor and Herta Steinkellner},
  journal={Biotechnology journal},
  year={2014},
  volume={9 4},
  pages={501-10}
}
Human butyrylcholinesterase (BChE) is considered a candidate bioscavenger of nerve agents for use in pre- and post-exposure treatment. However, the presence and functional necessity of complex N-glycans (i.e. sialylated structures) is a challenging issue in respect to its recombinant expression. Here we transiently co-expressed BChE cDNA in the model plant Nicotiana benthamiana with vectors carrying the genes necessary for in planta protein sialylation. Site-specific sugar profiling of secreted… CONTINUE READING

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