Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production

@article{Daly2005ExpressionOH,
  title={Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production},
  author={Rachel Daly and M. Hearn},
  journal={Journal of Molecular Recognition},
  year={2005},
  volume={18}
}
The use of the methylotrophic yeast, Pichia pastoris, as a cellular host for the expression of recombinant proteins has become increasing popular in recent times. P. pastoris is easier to genetically manipulate and culture than mammalian cells and can be grown to high cell densities. Equally important, P. pastoris is also a eukaryote, and thereby provides the potential for producing soluble, correctly folded recombinant proteins that have undergone all the post‐translational modifications… Expand
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