Expression of hepatitis C virus envelope glycoproteins by herpes simplex virus type 1-based amplicon vectors.

@article{Tsitoura2002ExpressionOH,
  title={Expression of hepatitis C virus envelope glycoproteins by herpes simplex virus type 1-based amplicon vectors.},
  author={Eliza Tsitoura and Michaela Lucas and Valerie Revol-Guyot and Alberto Luis Epstein and Roberto Manservigi and Penelope Mavromara},
  journal={The Journal of general virology},
  year={2002},
  volume={83 Pt 3},
  pages={561-6}
}
Herpes simplex virus type 1 (HSV-1)-based amplicon vectors expressing hepatitis C virus (HCV) E1 and E2 glycoproteins were investigated. HSV-1 amplicon vectors carrying the E1E2p7- or E2p7-coding sequences of HCV type 1a under the control of the HSV-1 IE4 (alpha22/alpha47) promoter were constructed. Studies of infected HepG2, WRL 68 or Vero cells indicated that HSV-1-based amplicon vectors express high levels of HCV glycoproteins that are processed correctly. Immunofluorescence microscopy… CONTINUE READING