Expression of hepatitis B surface antigen in transgenic banana plants

  title={Expression of hepatitis B surface antigen in transgenic banana plants},
  author={G. B. Sunil Kumar and Thumballi Ramabhatta Ganapathi and Chandu Revathi and Lingam Srinivas and Vishwas Bapat},
Embryogenic cells of bananan cv. [] Key Method The transgenic nature of the plants and expression of the antigen was confirmed by PCR, Southern hybridization and reverse transcription (RT)-PCR. The expression levels of the antigen in the plants grown under in vitro conditions as well as the green house hardened plants were estimated by ELISA for all the four constructs. Maximum expression level of 38 ng/g F.W. of leaves was noted in plants transformed with pEFEHBS grown under in vitro conditions, whereas…

Transient and stable expression of hepatitis B surface antigen in tomato (Lycopersicon esculentum L.)

The expression of the antigen in the fruits was confirmed by RT-PCR and ELISA analysis and is the first report on the expression of hepatitis B surface antigen in tomato.

Hepatitis B surface antigen expression in NT-1 cells of tobacco using different expression cassettes

The cell growth was optimum at lower concentrations of the antibiotics, and the maximum expression was noted at 200 mg dm−3 of kanamycin, while the effect of different concentrations of geneticin was studied on the growth of transformed cells and HBsAg expression.

Characterization of T 2 Tobacco Plants expressing the Hepatitis B gene for Molecular and Morphological Characters

It was found that the presence of the foreign gene did not inhibit the growth of the transgenic plants; transgenics showed similar growth rate compared to that of the control plants.

Expression of hepatitis B surface antigen (HBsAg) gene in transgenic cherry tomato

The feasibility of the expression of foreign antigens in cherry tomato plant for possible use as a raw and edible vaccine is indicated.

Analysis of the limitations of hepatitis B surface antigen expression in soybean cell suspension cultures

The supplementation of culture medium with a protease inhibitor, leupeptin hemisulfate could restore up to 50% of HBsAg expression in cell suspension cultures, the first report to investigate the possible cause and solution to the loss of recombinant protein expression levels in plant cell suspended cultures.

Expression of the Human Hepatitis B Virus Large Surface Antigen Gene in Transgenic Tomato Plants

This work suggests the possibility of producing a new alternative vaccine for human HBV through the expression of the modified HBV large surface antigen in transgenic tomato plants, the first time this gene has been expressed in plants.

Stability and inheritance analysis of transgenic Tobacco expressing Hepatitis B surface antigen

This phenotypic and molecular characterization strengthens the genetic stability and inheritance of the HBsAg gene in different generations of transgenic tobacco plants.

High-level production and aggregation of hepatitis B surface antigen in transgenic tobacco seeds

The HBsAg-expressing tobacco seeds could constitute a novel alternative source for the cost-effective production of the antigen, although seed-derived antigens sedimented slightly faster than the one produced in Pichia pastoris yeast.



Expression of hepatitis B surface antigen in tobacco cell suspension cultures.

Expression of hepatitis B surface antigen in transgenic plants.

Because the HBsAg produced in transgenic plants is antigenically and physically similar to theHBsAg particles derived from human serum and recombinant yeast, which are used as vaccines, it is concluded that transgenic Plants hold promise as low-cost vaccine production systems.

Immunogenicity of transgenic plant-derived hepatitis B surface antigen.

The immunological response elicited in vivo by using recombinant hepatitis B surface antigen (rHBsAg) purified from transgenic tobacco leaves was qualitatively similar to that obtained by immunizing mice with yeast-derived r HBsAg (commercial vaccine).

Production of hepatitis B surface antigen in transgenic plants for oral immunization

Mice fed transgenicHBsAg potato tubers showed a primary immune response that could be greatly boosted by intraperitoneal delivery of a single subimmunogenic dose of commercial HBsAg vaccine, indicating that plants expressing HBs Ag in edible tissues may be a new means for oral hepatitis B immunization.

Oral immunization with hepatitis B surface antigen expressed in transgenic plants

It is concluded that natural bioencapsulation of the antigen may provide protection from degradation in the digestive tract until plant cell degradation occurs near an immune effector site in the gut.

Factors Important in the Extraction, Stability and in Vitro Assembly of the Hepatitis B Surface Antigen Derived from Recombinant Plant Systems

Simple in vitro manipulations, compatible with the goal of a minimally processed oral vaccine, may prove valuable in increasing the immunogenicity of the plant‐derived antigen.

Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity

It has been demonstrated that ER retention is essential for the accumulation of sufficient scFv to bind high concentrations of ABA in the transgenic seeds and seed-specific expression of high amounts of anti-ABA-scFv's at a defined time of seed-development induced a developmental switch from seed ripening to vegetative growth.

Optimization of scFv antibody production in transgenic plants.

MSI-99, a magainin analogue, imparts enhanced disease resistance in transgenic tobacco and banana

The results suggest that MSI-99 can be useful in imparting enhanced disease resistance in transgenic plants and tobacco plants transformed with pMSI168 showed enhanced resistance against Sclerotinia sclerotiorum, Alternaria alternata and Botrytis cinerea.