Expression of hepatitis B surface antigen in transgenic banana plants

@article{Kumar2005ExpressionOH,
  title={Expression of hepatitis B surface antigen in transgenic banana plants},
  author={G. B. Sunil Kumar and Thumballi Ramabhatta Ganapathi and Chandu Revathi and Lingam Srinivas and Vishwas Bapat},
  journal={Planta},
  year={2005},
  volume={222},
  pages={484-493}
}
Embryogenic cells of bananan cv. [] Key Method The transgenic nature of the plants and expression of the antigen was confirmed by PCR, Southern hybridization and reverse transcription (RT)-PCR. The expression levels of the antigen in the plants grown under in vitro conditions as well as the green house hardened plants were estimated by ELISA for all the four constructs. Maximum expression level of 38 ng/g F.W. of leaves was noted in plants transformed with pEFEHBS grown under in vitro conditions, whereas…

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