Expression of growth factor receptors, the focal adhesion kinase, and other tyrosine kinases in human soft tissue tumors

  title={Expression of growth factor receptors, the focal adhesion kinase, and other tyrosine kinases in human soft tissue tumors},
  author={Timothy M. Weiner and Edison T. Liu and Rolf Joseph Craven and William Cance},
  journal={Annals of Surgical Oncology},
AbstractBackground: The tyrosine kinases are a family of genes that includes many growth factor receptors and protooncogenes. They appear to have a role in many cancers, but have not been systematically studied in the pathogenesis and progression of human sarcomas. Methods: To characterize the protein tyrosine kinases that are expressed in human sarcomas, we used a polymerase chain reaction (PCR)-based method to construct kinase-specific cDNA libraries from low-grade and high-grade primary… 
Focal adhesion kinase as a marker of invasive potential in differentiated human thyroid cancer
Overexpression of FAK may be part of a mechanism for invasion and metastasis of thyroid cancer, and the levels of p125FAK may serve as a marker of biologic behavior in this disease.
Focal adhesion kinase and cancer.
Immunohistochemical data of FAK expression and role in different cancer types tumors and discussed FAK inhibition therapy approaches are summarized.
Clinical significance of FAK expression in human neoplasia.
The purpose of this article is a comprehensive review of the existing data on FAK expression and signaling and their clinical significance in human malignancy.
Advances in Brief Overexpression of the Focal Adhesion Kinase ( p 125 @ K ) in Invasive Human Tumors '
Evidence is provided that both epithellal and mesenchymal tumor progression are accompanied by increased p125'@'@― expression and that the level of FAK expression might be a marker for the invasive potential of a tumor.
Focal Adhesion Kinase Overexpression in Endometrial Neoplasia
Upregulation of FAK is seen in both endometrial hyperplasia and carcinoma, implying that FAK may play an important role in endometrioid carcinogenesis and FIGO grade and p53 overexpression.
Inhibition of focal adhesion kinase decreases tumor growth in human neuroblastoma
The results of the current study suggest that FAK and phosphorylated at the Y397 site plays a role in neuroblastoma cell survival, and that the FAK Y397 phosphorylation site is a potential therapeutic target for this childhood tumor.
Focal adhesion kinase and p53 signaling in cancer cells.


Identification of a new endothelial cell growth factor receptor tyrosine kinase.
A new growth factor receptor tyrosine kinase (RTK) gene (designated KDR) has been cloned from a human endothelial cell cDNA library, and is expressed as a 7.0 kb transcript and localized to human chromosome 4.
Novel protein kinases expressed in human breast cancer
Different expression profiles in different primary breast carcinomas are revealed and may determine new molecular sub‐sets of human, which are determined by PCR cloning techniques based on consensus sequences in the kinase domain.
Expression of messenger RNAs for platelet‐derived growth factor and its receptors in human sarcoma cell lines
A large variability and independency in the expression of the 2 PDGF receptor types as well as of the genes encoding the corresponding ligands is shown.
axl, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase
Using a sensitive transfection-tumorigenicity assay, we have isolated a novel transforming gene from the DNA of two patients with chronic myelogenous leukemia. Sequence analysis indicates that the
Expression of platelet-derived growth factor and its receptors in proliferative disorders of fibroblastic origin.
Investigation of the occurrence of PDGF and PDGF receptors in a series of proliferative disorders of fibroblastic origin indicates that an autocrine growth stimulation via the PDGF beta-receptor could occur in an early phase of tumorigenesis, and may be a necessary but insufficient event for the progression into fully malignant human connective tissue lesions.
A novel endothelial cell surface receptor tyrosine kinase with extracellular epidermal growth factor homology domains
The cloning and characterization of tie is described, a novel type of human endothelial cell surface receptor tyrosine kinase that may have evolved for multiple protein-protein interactions, possibly including cell adhesion to the vascular endothelium.
pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.
The isolation of a cDNA encoding a protein, pp125, that is a major phosphotyrosine-containing protein in untransformed chicken embryo cells and exhibits an increase in phosphotYrosine in pp60v-src-transformedChicken embryo cells is reported.
Regulation of focal adhesion-associated protein tyrosine kinase by both cellular adhesion and oncogenic transformation
It is shown that tyrosineosphorylation of this protein is modulated both by cell adhesion and transformation by pp60v–src, and that these changes in phosphorylation are correlated with increased pp125FAKtyrosine kinase activity.
Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction.
  • A. Wilks
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1989
Two sequences were identified that, on the basis of sequence comparison with known PTKs, may encode representatives of a new class of PTK.
Altered expression of the retinoblastoma gene product in human sarcomas.
Tumors in which the expression of Rb gene product was decreased were more aggressive than tumors in which this protein was expressed by nearly all cells, suggesting the R b gene product may be an important prognostic variable in patients with these tumors.