Expression of green fluorescent protein in Lactococcus lactis.

@article{Palencia2000ExpressionOG,
  title={Expression of green fluorescent protein in Lactococcus lactis.},
  author={Pilar Fern{\'a}ndez de Palencia and Concepci{\'o}n Nieto and Paloma Acebo and Manuel Espinosa and Paloma Fern{\'a}ndez L{\'o}pez},
  journal={FEMS microbiology letters},
  year={2000},
  volume={183 2},
  pages={
          229-34
        }
}
The gfp gene from Aequorea victoria, encoding the green fluorescent protein (GFP) has been expressed in Lactococcus lactis subsp. lactis biovar cremoris MG1363, upon construction and introduction of plasmid pLS1GFP into this host. GFP was monitored in living cells during growth to evaluate its use in molecular and physiological studies. Quantification of the levels of GFP expressed by cultures was feasible by fluorescence spectroscopy. Phase-contrast and fluorescence microscopy allowed us to… CONTINUE READING

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