Expression of green fluorescent protein in Streptococcus gordonii DL1 and its use as a species-specific marker in coadhesion with Streptococcus oralis 34 in saliva-conditioned biofilms in vitro.

@article{Aspiras2000ExpressionOG,
  title={Expression of green fluorescent protein in Streptococcus gordonii DL1 and its use as a species-specific marker in coadhesion with Streptococcus oralis 34 in saliva-conditioned biofilms in vitro.},
  author={Marcelo B. Aspiras and K M Kazmerzak and Paul E. Kolenbrander and Roderick McNab and Neil J. Hardegen and Howard F Jenkinson},
  journal={Applied and environmental microbiology},
  year={2000},
  volume={66 9},
  pages={4074-83}
}
Streptococcus gordonii is one of the predominant streptococci in the biofilm ecology of the oral cavity. It interacts with other bacteria through receptor-adhesin complexes formed between cognate molecules on the surfaces of the partner cells. To study the spatial organization of S. gordonii DL1 in oral biofilms, we used green fluorescent protein (GFP) as a species-specific marker to identify S. gordonii in a two-species in vitro oral biofilm flowcell system. To drive expression of gfp, we… CONTINUE READING

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