Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins

@article{Martn2004ExpressionOG,
  title={Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins},
  author={Juan Francisco Mart{\'i}n and Santiago Guti{\'e}rrez and Francisco J. Fern{\'a}ndez and Javier Velasco and Francisco Fierro and Ana T Marcos and Katarina Kosalkov{\'a}},
  journal={Antonie van Leeuwenhoek},
  year={2004},
  volume={65},
  pages={227-243}
}
The genespcbAB,pcbC andpenDE encoding the enzymes (α-aminoadipyl-cysteinyl-valine synthetase, isopenicillin N synthase and isopenicillin N acyltransferase, respectively) involved in the biosynthesis of penicillin have been cloned fromPenicillium chrysogenum andAspergillus nidulans. They are clustered in chromosome I (10.4 Mb) ofP. chrysogenum, in chromosome II ofPenicillium notatum (9.6 Mb) and in chromosome VI (3.0 Mb) ofA. nidulans. Each gene is expressed as a single transcript from separate… 
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TLDR
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References

SHOWING 1-10 OF 106 REFERENCES
Cloning and Expression of the Fungal Expandase/hydroxylase Gene Involved in Cephalosporin Biosynthesis
TLDR
The cloned DAOCS/DACS gene represents an important tool for enhancing production of known cephalosporins and for synthesis of novel cep HALsporins.
Two genes involved in penicillin biosynthesis are linked in a 5.1 kb SalI fragment in the genome of Penicillium chrysogenum
TLDR
Two genes, pcbC and penDE, encoding the enzymes isopenicillin N synthase and acyl-CoA:6-amino penicillanic acid (6-APA) acyltransferase, which are involved in the penicillin biosynthetic pathway in Penicillium chrysogenum were cloned.
Cloning, characterization of the acyl-CoA : 6-amino penicillanic acid acyltransferase gene of Aspergillus nidulans and linkage to the isopenicillin N synthase gene
SummaryThe penDE gene encoding acyl-CoA:6-amino penicillanic acid acyltransferase (AAT), the last enzyme of the penicillin biosynthetic pathway, has been cloned from the DNA of Aspergillus nidulans.
Isolation, sequence determination and expression in Escherichia coli of the isopenicillin N synthetase gene from Cephalosporium acremonium
TLDR
The cloning, characterization and expression in Escherichia coli of the gene encoding the IPS protein in Cephalosporium acremonium is reported, and a new protein co-migrating with authentic IPS as the major protein of the cell.
Expression of the penDE gene of Penicillium chrysogenum encoding isopenicillin N acyltransferase in Cephalosporium acremonium: production of benzylpenicillin by the transformants
TLDR
Analysis of the hybridization patterns of the DNA of C. acremonium transformed with the pcbC or penDE genes indicated that integration occurs by non-homologous recombination.
Clusters of genes for the biosynthesis of antibiotics: Regulatory genes and overproduction of pharmaceuticals
  • J. Martín
  • Biology
    Journal of Industrial Microbiology
  • 2005
SummaryIn the last decade numerous genes involved in the biosynthesis of antibiotics, pigments, herbicides and other secondary metabolites have been cloned. The genes involved in the biosynthesis of
Characterization and expression in Streptomyces lividans of cefD and cefE genes from Nocardia lactamdurans: the organization of the cephamycin gene cluster differs from that in Streptomyces clavuligerus
TLDR
expression of both genes, cefD and cefE, in S. lividans transformants, results in deacetoxycephalosporin C synthase and isopenicillin N epimerase activities that are 10–12 times higher than those in N. lactamdurans and S. clavuligerus.
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