Expression of functional Na,K-ATPase isozymes in normal human cardiac biopsies.

@article{Lelievr2001ExpressionOF,
  title={Expression of functional Na,K-ATPase isozymes in normal human cardiac biopsies.},
  author={L G Lelievr and Gilles Crambert and P D Allen},
  journal={Cellular and molecular biology},
  year={2001},
  volume={47 2},
  pages={265-71}
}
In human heart failure, disturbances in Ca2+ homeostasis are well known but the fate of the Na,K-ATPase isoforms (alpha1beta1, alpha2beta1 and alpha3beta1), the receptors for cardiac glycosides, still remains under study. Microsomes have been purified from non-failing human hearts. As judged by the sensitivities of Na,K-ATPase activity to ouabain (IC50 values: 7.0 +/- 2.5 and 81 +/- 11 nM), 3H-ouabain-binding measurements at equilibrium with and without 10 mM K+ and by a biphasic ouabain… CONTINUE READING