Expression of enhancing factor gene and its localization in mouse tissues

  title={Expression of enhancing factor gene and its localization in mouse tissues},
  author={Rita Mulherkar and Shefali J. Desai and R. S. Rao and A. S. Wagle and Madhav G. Deo},
SummaryEnhancing factor (EF), a 14 kDa protein, isolated from mouse small intestines, has been reported from this laboratory. Based on our earlier studies EF has been implicated in cell proliferation. Preliminary immunohistochemical studies have shown EF to be localized in the Paneth cells of small intestines. In this paper we report the tissue distribution of EF using conditions optimized for immunohistochemical staining. In addition, the data are supported by northern blot analysis using a… 

Ontogeny of enhancing factor in mouse intestines and skin

This study of the ontogeny of EF shows that the appearance of the protein coincides with the appearing of mature Paneth cells of the small intestines, which means EF is associated with tissues which have a high growth rate.

Expression of enhancing factor/phospholipase A2 in ICRC mice

The molecular characterization of the ICRC mouse with respect to the enhancing factor gene is reported, demonstrating that EF from Balb/c and ICRC intestines are either immunologically identical or closely related to each other although, quantitatively, EF was very low in ICRC mice.

Trace amounts of enhancing factor/phospholipase A2 in mouse peritoneal exudate cells

The presence of trace amounts of EF in the peritoneal exudate cells could be a unique mode of transport of growth factor modulator to the site of injury to aid in regeneration/cell proliferation of damaged tissue.

Expression of enhancing factor/phospholipase A2 in skin results in abnormal epidermis and increased sensitivity to chemical carcinogenesis

Enhancing factor (EF), a growth factor modulator, is the mouse homologue of human secretory group II phospholipase A2. EF exhibits growth-promoting activity in vitro, in the presence of epidermal

Diversity in secreted PLA2-IIA activity among inbred mouse strains that are resistant or susceptible to ApcMin/+ tumorigenesis

A sensitive assay to directly quantitate sPLA2 activity in the murine intestinal tract utilizing a fluorescent BODIPY-labeled phospholipid substrate is developed, which enables further studies of the mechanisms of s PLA2 action influencing the development and tumorigenesis of the small intestine and colon in both mice and humans.

Expression of secretory phospholipase A2 in colon tumor cells potentiates tumor growth

The ability of sPLA2‐IIA to slow tumor cell growth is dependent upon the availability of substrate, and that in some instances sPLA1‐IIASWR may actually enhance tumor growth.

Influence of high-calorie (cafeteria) diets on the population of Paneth cells in the small intestine of the rat.

The effect of high-carbohydrate (HC) and high-fat (HF) cafeteria diets on the PC population in the small intestine of the adult rat is analyzed and a multifactorial control of PC density is proposed.

Colon cancer stem cells.

Stem cell biology in the context of CRC is focused on to help understand the mechanisms leading to tumor development and therapy resistance, with possible therapeutic indications.

The roles of sPLA 2-IIA ( Pla 2 g 2 a ) in cancer of the small and large intestine

SPLA2-IIA protects the mucosa from bacterial damage and prevents chronic inflammation and maintains a balance between Wnt, Notch and other key signaling pathways, and promotes apoptosis of cancer cells.



Immunohistochemical localization of epidermal growth factor in rat and man

SummaryEpidermal growth factor (EGF) is a peptide which stimulates cell mitotic activity and differentiation, has a cytoprotective effect on the gastroduodenal mucosa, and inhibits gastric acid

Further studies on the enhancing factor and its possible mechanism of action

The role of EF may be to trap EGF and make it available to the cells through its own receptors even in the absence of EGF receptors, which is distinct from the receptor for epidermal growth factor.

The Paneth cell: a source of intestinal lysozyme.

The results demonstrate that the lysozyme of the succus entericus is, at least in part, derived from the Paneth cell, and is probably present in thePaneth cell granules, and its secretion is stimulated by pilocarpine.

Intestinal epithelial differentiation: new insights from chimeric and transgenic mice

  • J. Gordon
  • Biology
    The Journal of cell biology
  • 1989
The purpose of this mini-review is to emphasize the usefulness of these powerful methods for examining questions related to enteric epithelial biology.

Complementary DNA cloning and sequencing of rat ovarian basic fibroblast growth factor and tissue distribution study of its mRNA.

Paneth-cell function.

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels

Molecular Cloning: A Laboratory Manual

The content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.

Immunohistochemical localization of 'enhancing factor', a novel growth modulator, in the mouse small intestines.