Expression of enhanced levels of small RNA polymerase III transcripts encoded by the B2 repeats in simian virus 40-transformed mouse cells

@article{Singh1985ExpressionOE,
  title={Expression of enhanced levels of small RNA polymerase III transcripts encoded by the B2 repeats in simian virus 40-transformed mouse cells},
  author={Karambir Singh and Michael F. Carey and Sentob Saragosti and Michael R. Botchan},
  journal={Nature},
  year={1985},
  volume={314},
  pages={553-556}
}
Although specific viral genes are known which are sufficient to transform certain types of cells, viral transformation has been shown to he accompanied by a change in the level of expression of cellular genes. Assuming that this latter class of genes is involved in some way in the establishment of the transformed state, we and others1,2 have sought to characterize them. To this end, we constructed a complementary DNA library from a simian virus 40 (SV40)-transformed mouse cell line and screened… 
Induction of specific transcription by RNA polymerase III in transformed cells
RNA polymerase III (pol III) transcripts of the highly repeated mouse B2 gene family are increased in many oncogenically transformed murine cell lines. In cells transformed by simian virus 40, the
Elevated amounts of a 7S nuclear RNA with sequence homology to a tumor virus promoter in transformed and tumorigenic cells.
TLDR
Results suggest that this particular RNA may be involved in stimulating transcription of some cellular oncogenes in tumor tissues, and is not dependent on the presence of SV 40 sequences.
Enhanced B2 transcription in simian virus 40-transformed cells is mediated through the formation of RNA polymerase III transcription complexes on previously inactive genes.
  • M. Carey, K. Singh
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1988
TLDR
It is demonstrated that the augmented expression results from the formation of pol III transcription complexes on previously inactive B2 genes, and extracts prepared from normal growing cells and transformed cells transcribed cloned pol III templates 5-fold more efficiently than extracts from normal confluent cells.
Multiple Mechanisms Contribute to the Activation of RNA Polymerase III Transcription in Cells Transformed by Papovaviruses*
TLDR
Both papovaviruses stimulate pol III transcription by boosting production of selected components of the basal machinery by activating several signal transduction pathways in fibroblasts transformed by polyomavirus or simian virus 40.
The rodent B2 sequence can affect expression when present in the transcribed region of a reporter gene.
TLDR
Although the B2 sequences affected expression of the reporter gene, these effects did not appear to be due to hybridization of the small B2 RNA to the reporter transcripts, suggesting these repetitive elements may be cis-acting regulators of gene expression.
Specific induction of cellular gene transcription in herpes simplex virus type 2-transformed cells.
In spite of much work, the mechanism of oncogenic transformation by herpes simplex virus (HSV) is as yet unknown. It has been proposed that HSV type 2 (HSV-2) can transform cells by a 'hit and run'
Isolation of cellular genes differentially expressed in mouse NIH 3T3 cells and a simian virus 40-transformed derivative: growth-specific expression of VL30 genes
We constructed and screened a cDNA library made from simian virus 40 (SV40)-transformed NIH 3T3 cells, and we isolated cDNAs representing genes that are differentially expressed between the parental
Synthesis and processing of small B2 transcripts in mouse embryonal carcinoma cells
B2 genes are short repeated sequences which are transcribed by RNA polymerase III. Abundant transcripts accumulate in embryonic and transformed cells, but transcripts are rare or absent from normal
Transcriptional regulation of two serum-induced RNAs in mouse fibroblasts: equivalence of one species to B2 repetitive elements
We obtained eight cDNA clones that define five genes whose expression (appearance of transcripts in the cytoplasm) is enhanced when quiescent mouse fibroblasts are stimulated with serum to divide.
Increased levels of B1 and B2 SINE transcripts in mouse fibroblast cells due to minute virus of mice infection.
TLDR
The effect of MVM infection on host cell gene expression in mouse fibroblast cells (LA9 cells), analyzed by differential display, reveals that few cellular protein-coding genes appear to be up- or downregulated and identifies the murine B1 and B2 short interspersed element (SINE) transcripts as being increased upon M VM infection.
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  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1974
TLDR
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